Downregulation of microRNA-34 induces cell proliferation and invasion of human mesothelial cells
4.6 (206) In stock
Malignant mesothelioma (MM) is an aggressive tumor with a dismal prognosis, and the molecular alterations involved in this disease remain unknown. We previously reported that microRNA-34s (miR-34s) are methylated and downregulated in MM and may play an important role in the carcinogenesis of MM. In this study, we downregulated miR-34s in human mesothelial cells to investigate the cellular effect of miR-34 knockdown. For the cell study, we used LP-9, a human mesothelial cell line, and three human primary-cultured mesothelial cell lines. RNA-based miR-34a, -34b and -34c inhibitors were transfected into these cells, and their effects on proliferation and invasion were evaluated. A scramble RNA oligonucleotide was used as a control. The protein expression status was estimated using western blotting. After miR-34 inhibitor transfection, miR-34a, -34b and -34c were downregulated in all the examined mesothelial cell lines. miR-34 inhibitor transfection significantly increased cell proliferation in all of the mesothelial cell lines, compared with the scramble control. The invasive ability also increased in the miR-34 inhibitor transfectants, compared with the scramble control, in the LP-9 cell line. Western blotting confirmed the upregulation of c-MET, phospho-c-MET, and bcl-2 proteins in LP-9 cells after miR-34 inhibitor transfection. In conclusion, our study showed that the downregulation of miR-34s induced an oncogenic phenotype in non-malignant mesothelial cells. The present study, together with the results of our previous report, strongly suggest that miR-34s play an important role in the early carcinogenic process involved in the transformation of human mesothelial cells to MM.
The clinical impact of intra‐ and extracellular miRNAs in ovarian
Evaluation of miRNA-derived Extracellular Vesicles as Biomarkers
Frontiers MicroRNAs: Pleiotropic Regulators in the Tumor
MicroRNAs as potential biomarkers in malignant pleural mesothelioma
The role of Exosomal miRNAs in cancer
Biomedicines, Free Full-Text
A polymorphism site in the pre‑miR‑34a coding region reduces miR‑34a expression and promotes osteosarcoma cell proliferation and migration
PDF) Abstract 187: Down-regulation of microRNA34 induces cell
MicroRNAs target the Wnt/β‑catenin signaling pathway to regulate
A comparison between the effects of over-expression of miRNA-16 and miRNA-34a on cell cycle progression of mesothelioma cell lines and on their cisplatin sensitivity - ScienceDirect
Active Secretion of Dimerized S100A11 Induced by the Peroxisome in Mesothelioma Cells
Frontiers Approaches Toward Targeting Matrix Metalloproteases
Victoria's Secret, Intimates & Sleepwear
miR-34 loci and expression levels of miR-34a, miR-34b, miR-34b
Curve Muse Plunge Bralette with Floral Lace-2pack-PINK,MAUVE-M:34A 34B 34C 36A 36B 36C
MiR-34a and miR-34b/c have distinct effects on the suppression of lung adenocarcinomas
Shein Womens Black Solid Underwire Bandeau Bra Padded Size Small
- Maquina multifuncional para madera MQ443A
- Lingerie Solutions Women's Water Push-up Pads Nude Bra Inserts
- Invasão SW - LineBR (Bônus: 100k Bonittao)
- Buy Pink Adjustable Waist Ruched Leggings online in Dubai
- Speed Boost belly drum Kommo-O is not the most broken Pokemon
- Gyarados GX ULTRA RARE SM212 Hidden Fates Tin Promo Card Pokemon TCG NM Holo